What is the purpose of the jis z 2801 test method in the laboratory?
The JIS Z 2801 is one of the test methods designed to test the hard surfaces quantitatively to inhibit the growth of the microorganism. The jis z 2801 is approved as the IOS (International Organization for Standardization) Procedure. With the help of this article, you will know about the features, summary, strengths, weaknesses, and description of the JIS Z 2801 test method.
What are the features of the JIS Z 2801 test method?
The key features of the jis z 2801 test method are given by,
- The International Standard Organization (ISO) which is used to specify a quantitative testing method to determine the antibacterial activity of the textile products, including the non-woven fabric.
- It is suitable for all kinds of textile products such as fabric, wadding, thread, and material for clothing, home decoration, and various products regardless of the type of antimicrobial agent used (organic or inorganic natural or artificial) or method of use ( built-in after treatment or organ transplantation).
- It depends on the anticipated use and the environment in which the textile product is intended to be used.
- The customer can select the appropriate method for determining the antimicrobial activity. The different types of procedures in the microbial test. They are absorption Method, assessment Method, bacterial suspensions are directly inoculated on the sample, colony plate count method, and adenosine Triphosphate luminescence method are also indicated for quantifying the enumeration of bacteria.
What is the summary of the JIS Z 2801 test?
The summary of the jis z 2801 test is given by,
First, the test microorganisms are usually prepared by growing in a liquid culture medium.
Then the microbial test suspensions were normalized by dilution in nutritious broth. (This gives the microbe the potential to grow during the test.)
The control and test surfaces were triple injected with microorganisms. The inoculums were then covered with a sterile thin film. The inoculation will spread on the surface of the sample to prevent evaporation and provides the close contact with the antimicrobial surface.
The microbial concentration was determined at the zero time by elution which is followed by dilution and plating.
Controls are carried out to determine whether the neutralization/washing method effectively neutralizes the antimicrobial agent in the antimicrobial surface being tested.
Inoculated surface cover control and the antimicrobial test allowed incubating undisturbed eggs in a humid environment for 24 hours.
After incubation, the concentration of microorganisms is determined.
Finally, the calculation between the microbial reduction vs. initial concentration and control surface.
What is the strength of the JIS Z 2801 test?
The strength of the jis z 2801 test are given by,
This approach is used to detect the quantitative, and results tend to be reproducible. If the inoculums are not spill over the target area after coating with a thin film.
This type of method is used for testing both the properties of bacteria (Inhibit growth) and kill bacteria (kill bacteria).
In this test method, the concentration of microorganisms was standardized, and the bacteria will receive nutrients during the incubation period. This provides ample opportunity for growth if the substrate does not contain enough antimicrobial compounds. This is in contrast to other antimicrobial tests in which the microorganisms are “incubated” in a non-nutrient suspension, which can be stressful in the long run.
This method defines a triple experiment. This allows researchers to assess the accuracy of individual tests and increase overall experimental accuracy.
This method includes the criteria “Pass/Fail” for the calculated level of antimicrobial activity observed in the test sample. This makes the determination of antimicrobial activity less discretionary.
What is the weakness of the JIS Z 2801 test?
The weakness of the JIS Z 2801 test is given by,
The JIS Z 2801 method does not necessarily represent the actual contamination surface. This is because of the relatively dilute liquid microorganisms spread over a large surface area then wet. In most cases, microbial contaminants dry quickly on the surfaces. This limits the amount of time the aqueous medium is available to facilitate interaction between antimicrobial and microbial characters. This means that the JIS Z 2801 is the “best case” test for many products.
It is not uncommon for “control” plastics to exert low levels of antimicrobial activity. Therefore, using this test method to identify the ideal control surface can be difficult.
What is the description of the JIS Z 2801 test?
The description of the JIS Z 2801 test is given by,
The test organisms to be used were two strains of Escherichia coli (ATTC 25922/ATTC 8739) and two strains of Staphylococcus aureus (ATTC 6538/ATTC 6538p) that were grown at 37˚C for 24 h and diluted to contain phenoxyethanol and the working concentration 5 x 105 cells/ml.
Then control and test surfaces were inoculated with work cultures and coated with a sterile film to ensure close homogeneous contact surfaces.
The survival of bacteria at the starting point (t = 0) was determined by serial dilution and coating on the appropriate agar. At the same time, the untreated and treated samples were incubated at 37°C for 18 h.
The sample is washed to separate bacteria from the surface. The solution for this extraction was subsequently diluted and coated on suitable agar to identify the colony-forming units.
The control group examined the elution method to ensure that the antimicrobial neutralization was effective.
The efficacy of antimicrobial agents was determined by calculating the log reduction in bacterial viability produced by the test samples.
From the above discussion, you can get a clear idea about the JIS Z 2810 test method. There are different types of antimicrobial tests, but this emerges as the industry standard.
Also read - What does ISO 20743 test method mean for a common textile businessman.
