What You Need To know about the JIS z 2801 test method.

Microbe Investigations AG (MIS) provides reliable, fast, affordable product development and quality control.

JIS stands for the Japanese Industrial Standard.

The JIS Z 2801 is one of the test methods designed to check the hard surfaces quantitatively to inhibit the expansion of the microorganism. The jis z 2801 is approved because of the IOS (International Organization for Standardization) Procedure. With this article’s assistance, you’ll realize the characteristics, summary, benefits, disadvantages, and outline of the jis z 2801 test method.

What are the aspects of the JIS Z 2801 test?

The aspects of the jis z 2801test method are given by,

Japanese Industrial Standard (JIS) Z 2801 is a commonly requested quantitative antimicrobial test for plastics, foams, and textiles and is compliant with ISO 22196.

The JIS Z 2801 standard method tests hard surfaces and plastics to measure potential growth. Climb After inoculation by two common bacteria, E. coli and S. aureus, the usual test is 24 hours. JIS Z 2801 is one of the most frequently requested, rapid, and reliable antimicrobial surface tests for its antibacterial activity and efficacy.

Durability testing in conjunction with JIS Z 2801 can provide important information about the antimicrobial performance of products when exposed to different environmental conditions such as heat, humidity, UV, etc.

As with all antimicrobial tests in the laboratory recommends first complying with the requirements of the end customer and relevant regulatory authorities when choosing a test method.

What are the advantages of the JIS Z 2801 test method?

The advantages of the JIS Z 2801 test method are given by,

• JIS Z 2801 was developed for materials and products entering the Japanese market.
• The JIS standard is a trusted and recognized worldwide antimicrobial test.
• The JIS standard is aligned for a wide range of markets.
• JIS Z 2801 harmonized version, ISO 22196 is almost the same as JIS Z 2801.

At Situ Biosciences product testing laboratories, we place great emphasis on high-quality testing. He believes that a thorough understanding of project requirements and communication is the main focus of every project.

Either choose the right testing method or set up a quality control program. Our experienced technical and commercial staff can assist and initiate necessary product testing. Using the Situ Biosciences antimicrobial laboratory for fast and reliable antimicrobial testing can support product development and efficacy testing as well as continuous quality control.

What are the steps involved in the JIS Z 2801 test method?

The steps are involved in the jis z 2801 test method is given by,

Step 1: Prepare sample

First, you can prepare the sample for the antimicrobial test. The samples are fabric, cloth, plastic, and other components. The sample size is 5cmx5cm, and two-part of the test sample and six parts of the untreated sample.

Step 2: Sterilization

The second step in the antimicrobial JIS Z 2801 test is sterilization. In the sample, you can add 70% of ethanol which is used to sterilize the sample.

Step 3: Inoculation:

For the inoculation process, you can add the S aurous and E coli on the top of the sample and then take the clear film of 4cmx4cm, which is used to cover the sample completely.

Step 4: Incubation

The incubation process will take on the sample after 24 hours. You can rest the sample after the inoculation process.

Step 5: Wash and shake.

The next step is washing and shaking of the sample. You can use 10ml of SCDLP liquid on the glass jar. And then, the sample will be taken and put it on the glass jar to wash and then shake the sample with the liquid.

Step 6: Counting

The final step in this test method is counting. You can place the sample on the agar plates and measure the sample. You can compare the count of the treated sample and the untreated sample. As a result, you can see the count difference between the treated and untreated samples.

Summary steps for the JIS Z 2801 test method:

• The microorganisms tested were normally prepared by growing in a liquid culture medium.
• Test microbial suspensions were normalized by dilution in nutritious broth (this allow microorganisms to propagate during testing)
• The control and test surfaces were inoculated with microorganisms. Then the organism, the leavening agent, is covered with a thin, sterile film. The cover of the leavening agent will spread to prevent it.
• Evaporates and ensures close contact with the antimicrobial surface.
• The concentration of microorganisms was determined at “zero time” by erosion, followed by dilution and jelly.
• The use of vaccinated surfaces is permitted cover control and antimicrobial testing to hatch undisturbed eggs in a humid environment for 24 hours, usually at body temperature.
• After incubation, the concentration of microorganisms is determined. reduction of microorganisms calculated against the control surface

Scientific Prevention Criteria of JIS Z 2801:

Must meet the following criteria are given by,

1. The average number of viable bacteria recovered from the time required for a sample was zero. Approx. 1 x 105 cells/carrier or higher
2. Consistency between repetitions must be observed for zero sample time.
3. The number of live bacteria recovered from the control surface after contact time.
4. Must not be significantly lower than the original inoculums concentration (>2-Log10).
5. Positive/growth controls shall demonstrate the growth of appropriate test microorganisms.
6. Negative/purity controls must not show the growth of test microorganisms.

Passing criteria of the test method:

JIS establishes efficacy criteria for antimicrobial efficacy greater than or equal to 2 Log10 or test microorganisms were reduced by 99% when comparing treated surfaces with controls surfaces after the exposure time. Alternatively, the passing criteria may be determined by the study.

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